IFN gamma protein (30R-1053)

Purified recombinant Human IFN gamma protein

Synonyms Ifng protein, Interferon gamma precursor protein, Immune interferon protein, T Cell interferon protein, IFN gamma protein, Interferon gamma protein, IFNG protein, Interferon-g protein, IFN-gamma protein, Type II Interferon protein, IFN immune protein, MAF protein, IFG protein, IFI protein, , Macrophage activating factor protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of IFN gamma protein (30R-1053)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ
Expression System E.coli
Purity > 95% pure
Molecular Weight 16.9 kDa (144aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in PBS, pH 7.4.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Human interferon-gamma (hIFN-gamma) is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Natural human interferon-gamma is composed of 143 amino acid residues without cysteine residues and is glycosylated. Recombinant human interferon-gamma was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Additional amino acid (Methionine) is attached at N-terminus.

References
Add a Paper

Lam Raga A. Markelya, Boon Tee Ongb, Kong Meng Hoib, Gavin Teob, Michelle Y. Lua, Daniel I.C. Wang

A high-throughput method for quantification of glycoprotein sialylation

Analytical Biochemistry

Volume: 407 Issue: 1 Page: 128-133 DOI: 10.1016/j.ab.2010.07.029

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Images

  • Coomassie Blue stained SDS-PAGE of IFN gamma protein (30R-1053) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $180.00
Size: 100 ug
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