IFN gamma protein (30R-AI069)

Purified recombinant Human IFN gamma protein

Synonyms IFN g protein, Immune Interferon protein, T cell interferon protein, IFN-g protein, MAF protein, type II interferon protein, Interferon gamma protein
Species Human
Protein Type Recombinant
Applications User optimized

Specifications

Residues Region of IFN Gamma protein corresponding to amino acids MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC.
Expression System E.coli
Purity > 98% pure
Molecular Weight 15.6 kDa
Form & Buffer Lyophilized from 10mM Na3PO4, pH7.2. Reconstitute in 10 mM Na3PO4 pH7.2 to a concentration of 0.1-1.0 mg/ml. When dissolved, dilute further into aqueous buffered solutions.

Usage & Assay Information

Bioactivity The ED50 as determined by a cytopathic affect inhibition assay with Mouse L929 cells challenged with EMC virus was 1 x 10^7 units/mg.

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma is species-specific and is biologically active only in human and primate cells.

References
Add a Paper

Lam Raga A. Markelya, Boon Tee Ongb, Kong Meng Hoib, Gavin Teob, Michelle Y. Lua, Daniel I.C. Wang

A high-throughput method for quantification of glycoprotein sialylation

Analytical Biochemistry

Volume: 407 Issue: 1 Page: 128-133 DOI: 10.1016/j.ab.2010.07.029

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Availability: In stock

Price: $225.00
Size: 100 ug
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