IL1 beta protein (30R-AI003)

Purified recombinant Human IL1 beta protein

Synonyms IL1, Leukocyte Endogenous Mediator protein, Lymphocyte-activating factor protein, LAF protein, Mononuclear Cell Factor protein, MCF protein, Catabolin protein, IL 1, IL-1 protein, Interleukin 1 beta protein, IL 1 protein, Endogenous Pyrogen protein, EP protein, LEM protein, IL-1 beta protein, IL-1
Species Human
Protein Type Recombinant
Applications User optimized

Specifications

Residues Region of IL1 beta protein corresponding to amino acids APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS.
Expression System E.coli
Purity > 98% pure
Molecular Weight 17.3 kDa
Form & Buffer Sterile filtered through a 0.2 um filter. Lyophilized from 10 mM Tris, pH 8.2. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. When dissolved, dilute further into aqueous buffered solutions.

Usage & Assay Information

Bioactivity The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Mouse D10S cells is approximately 1.0 pg/ml, corresponding to a specific activity of approximately 1 x 10^9 units/mg.

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance IL1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL1a and IL1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL2 release, B-cell maturation and proliferation, mitogenic FGFlike activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL1beta is a secreted cytokine, IL1a is predominantly a cell-associated cytokine.

References
Add a Paper

M Proulx, M Safoine, D Mayrand, K Aubin, A Maux, J Fradette

Impact of TNF and IL-1β on capillary networks within engineered human adipose tissues

Journals of Materials Chemistry B

Volume: 4 (2016) Page: 3608-3619 DOI: 10.1039/C6TB00265J

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Availability: In stock

Price: $225.00
Size: 10 ug
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