IL15 protein (His tag) (80R-1036)

Purified recombinant Human IL15 protein

Synonyms IL15, Interleukin-15 protein, IL-15, IL 15, IL15, IL-15 protein, IL 15 protein, IL-15 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information Cell line: CTLL2 (mouse cytotoxic Tcell) Maintenance Condition: 10% FBS RPMI 1640 with hIL2 Assay Medium: 10% FBS RPMI 1640 without hIL2 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation Time: 48 hr (after sample treatment) Concentration Range: 0.039 ng/ml - 40 ng/ml Detection Method: MTT assay

Images

Coomassie Blue stained SDS-PAGE of IL15 protein (His tag) (80R-1036)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 20-152 amino acids: MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTSLEHHH HHH
Expression System E.coli
Purity > 95% pure
Molecular Weight 13.9 kDa (123aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in PNS, pH 7.4, containing 0.2mM PMSF, and 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is < 2.5 ng/ml. Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Interleukin (IL-15) is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its participation in a trans signaling mechanism in which IL-15 receptor alpha (IL-15R alpha) from one subset of cells presents IL-15 to neighboring IL-2R beta/gamma(c)-expressing cells. This newly discovered cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells. Recombinant human IL-15 was expressed in E. coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.

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Images

  • Coomassie Blue stained SDS-PAGE of IL15 protein (His tag) (80R-1036) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $340.00
Size: 100 ug
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