IL2 antibody (10R-8673)
Mouse monoclonal IL2 antibody
|Synonyms||Monoclonal IL2 antibody, IL2 antibody, IL-2 antibody, TCGF antibody, Aldesleukin antibody, T-cell growth factor antibody, Interleukin-2 antibody, IL-2 antibody, interleukin 2 antibody|
|Immunogen||IL2 antibody was raised in Mouse using recombinant human IL-2 (22-153aa) purified from E. coli as the immunogen|
Western Blot analysis using IL2 antibody (10R-8673)
The cell lysates of Ramos (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with IL-2 antibody (1:500).
|Method of Purification||IL2 antibody was purified by protein-A affinity chromatography|
|Form & Buffer||Supplied in liquid form in Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol and 0.02% Sodium Azide|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:500, WB: 1:500|
Storage & Safety
|Storage||Can be stored at 4 deg C short term. For long term storage, aliquot and store at -20 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||IL-2 (Interleukin 2) is 153 amino acid protein. IL-2 produced by T cells in response to antigenic or mitogenic stimulation. This protein is required for T cell proliferation and other activities crucial to regulation of the immune response. IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. Three different IL-2 dependent signal transduction pathways have been identified: the c-Fos/c-Jun induction pathway mediated by Src family protein-tyrosine kinases; the c-Myc induction pathway; and the Rapamycin-sensitive pathway.|
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