IL2 protein (30R-1070)

Purified recombinant Human IL2 protein

Synonyms T-cell growth factor protein, Interleukin 2 protein, IL-2, IL-2 protein, IL 2 protein, IL-2 protein, Aldesleukin protein, Interleukin-2 protein, IL 2, IL2, IL 2 protein, TCGF protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information Cell line: CTLL2 (mouse cytotoxic T-cell) Maintenance Condition: 10% FBS RPMI 1640 with hIL2 Assay medium: 10% FBS RPMI 1640 without hIL2 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation Time: 24hr (after sample treatment) Concentration Range: 0.039 ng/ml - 40 ng/ml Detection method: MTT assay

Images

Coomassie Blue stained SDS-PAGE of IL2 (C145S) protein (30R-1070)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFSQSIIS TLT
Expression System E.coli
Purity > 95% pure
Molecular Weight 15.4 kDa (133aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in PBS, pH 7.4.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1.5 ng/ml.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance IL-2, also known as T cell growth factor(TCGF), is an immunoregulatory lymphokine produced by T-cells in response to protein or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. This recombinant human IL-2 is a 15.4 kDa protein, containing 133 amino acid residues including one intra chain disulfide bond. Recombinant human IL-2 was overexpressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

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Images

  • Coomassie Blue stained SDS-PAGE of IL2 (C145S)  protein (30R-1070) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $340.00
Size: 100 ug
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