IL3 protein (His tag) (80R-1035)

Purified recombinant Human IL3 protein

Synonyms interleukin 3 (colony-stimulating factor protein, P-cell stimulating factor protein, IL-3 protein, multiple) protein, IL-3 protein, MCGF protein, IL 3 protein, hematopoietic growth factor protein, Mast cell growth factor(MCGF) protein, IL-3, mast-cell growth factor, Multipotential colony-stimulating factor(multi-CSF) protein, IL3, IL 3, multilineage-colony-stimulating factor protein, MULTI-CSF protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information Cell line: TF1 (human erythroleukemic cell) Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF Assay medium - 10% FBS RPMI 1640 without Gm-CSF Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) Starvation: 24hr, Assay medium Incubation time: 24 hr (after sample treatment) Concentration range: 0.048 ng/ml - 25 ng/ml Detection method: MTT assay

Images

Coomassie Blue stained SDS-PAGE of IL3 protein (His tag) (80R-1035)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues MGSSHHHHHH SSGLVPRGSH MAPMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
Expression System E.coli
Purity > 90% pure
Molecular Weight 17.3 kDa (154aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris, pH 8.0, containing 0.2mM PMSF, and 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Interleukin-3 (IL-3) stimulates the proliferation of hematopoietic pluripotent progenitor cells. It is secreted by activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. IL-3 exerts its biological activities through binding to specific cell surface receptors. IL-3 receptor is a complex consisting of alpha and beta subunits, which in turn induces activation of JAK2/ STAT5, induction of c-myc, and activation of the Ras pathway. Human IL3 is a protein of 15-17 kDa (133 amino acids). It is synthesized as a precursor containing a hydrophobic secretory signal sequence of 19 amino acids. IL3 contains two putative glycosylation sites at positions 15 and 70 and contains a single disulfide bond (Cys16/84). Recombinant human IL-3 was expressed in E. coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.

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Images

  • Coomassie Blue stained SDS-PAGE of IL3 protein (His tag) (80R-1035) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $340.00
Size: 100 ug
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