Involucrin antibody (BSA/Azide Free) (10R-I148AP)

Mouse monoclonal Involucrin antibody (BSA/Azide Free)

Synonyms Monoclonal Involucrin antibody, Anti-Involucrin antibody
Cross Reactivity Dog, human, gorilla, monkey, owl, pig, rat
Applications ELISA, IF, IHC-F, IHC-P, IP
Immunogen Involucrin antibody (BSA/Azide Free) was raised in mouse using human keratinocytes? involucrin as the immunogen.

Images

Immunohistochemical staining using Involucrin antibody (BSA/Azide Free) (10R-I148AP)

Formalin-fixed, paraffin-embedded human skin stained with Involucrin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining in the epidermal region.

Specifications

Host Mouse
Clone SY5
Isotype IgG1
Method of Purification Involucrin antibody (BSA/Azide Free) was purified by Protein G chromatography.
Molecular Weight 66-170 kDa (predicted detection band MW)
Form & Buffer Antibody purified from ascites fluid by protein G chromatography and supplied in 10mM PBS, pH 7.4.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations IHC (f/p): 1:100-1:200, IP: 2 ug/mg protein lysate

Storage & Safety

Storage Store at 4 deg C.

General Information

Biological Significance Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

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Images

  • Immunohistochemical staining using Involucrin antibody (BSA/Azide Free) (10R-I148AP) | Formalin-fixed, paraffin-embedded human skin stained with Involucrin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining in the epidermal region.

Availability: In stock

Price: $618.20
Size: 200 ug
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