ISCU protein (His tag) (80R-1602)

Purified recombinant Human ISCU protein

Synonyms Iron-sulfur cluster assembly enzyme ISCU protein, NIFU protein, NIFUN, HML protein, hnifU protein, ISU2 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of ISCU protein (His tag) (80R-1602)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 35-167 amino acids: MGSSHHHHHH SSGLVPRGSH MYHKKVVDHY ENPRNVGSLD KTSKNVGTGL VGAPACGDVM KLQIQVDEKG KIVDARFKTF GCGSAIASSS LATEWVKGKT VEEALTIKNT DIAKELCLPP VKLHCSMLAE DAIKAALADY KLKQEPKKGE AEKK
Expression System E.coli
Purity > 90% pure
Molecular Weight 16.7 kDa (154aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT and 100mM NaCl.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Iron-sulfur cluster assembly enzyme, also known as ISCU, is a member of the nifU family. Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. It is interact with ISCS, a cysteine desulfurase, to sequester inorganic sulfur for Fe-S cluster assembly. ISCU-ISCS protein complex localizes in both mitochondria and cytosol, implying that Fe-S cluster assembly takes place in multiple subcellular compartments in mammalian cells. Recombinant human ISCU protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of ISCU protein (His tag) (80R-1602) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 10 ug
OR
Shipping
View Our Distributors