JAK3 antibody (10R-1874)

Mouse monoclonal JAK3 antibody

Synonyms Monoclonal JAK3 antibody, Anti-JAK3 antibody, JAK-3 antibody, JAK 3 antibody, Janus kinase 3 antibody, JAK3, JAK 3, JAK-3, JAK 3 antibody, JAK-3 antibody
Cross Reactivity Human, Mouse
Applications ELISA, FC, IF, WB
Immunogen JAK3 antibody was raised in Mouse using a purified recombinant fragment of human JAK3 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using JAK3 antibody (10R-1874) (JAK3 antibody)

FC analysis of Hela cells using JAK3 antibody (blue) and negative control (red).

Specifications

Host Mouse
Clone 5H2
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, FC: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance JAK3 is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease).

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Images

  • Flow Cytometric assay using Western Blot analysis using JAK3 antibody (10R-1874) (JAK3 antibody) | FC analysis of Hela cells using JAK3 antibody (blue) and negative control (red).
  • Western Blot analysis using JAK3 antibody (10R-1874) | Western Blot showing JAK3 antibody used against Jurkat cell lysate (1).
  • Immunofluorescent staining using Western Blot analysis using JAK3 antibody (10R-1874) (JAK3 antibody) | Confocal immunofluorescence analysis of Hela (left) and HepG2 (right) cells using JAK3 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
  • Immunofluorescent staining using Western Blot analysis using JAK3 antibody (10R-1874) (JAK3 antibody) | Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Availability: In stock

Price: $285.00
Size: 100 ul
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