Keratin K18 antibody (10R-2034)

Mouse monoclonal Keratin K18 antibody

Synonyms Monoclonal Keratin K18 antibody, Anti-Keratin K18 antibody, Keratin K 18, Keratin K18, Cytokeratin 18 antibody, Keratin K-18, Keratin K-18 antibody, Keratin K 18 antibody
Cross Reactivity Human
Applications ELISA, IHC, WB
Immunogen Keratin K18 antibody was raised in Mouse using a purified recombinant fragment of human Keratin K18 (aa391-483) expressed in E. coli as the immunogen.

Images

Immunohistochemical staining using Keratin K18 antibody (10R-2034)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), hepatocarcinoma (B), stomach cancer (C) and colon cancer tissue (D), showing cytoplasmic location with DAB staining using CK18 antibody.

Specifications

Host Mouse
Clone 4D11E4
Isotype IgG2b
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

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Images

  • Immunohistochemical staining using Keratin K18 antibody (10R-2034) | Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), hepatocarcinoma (B), stomach cancer (C) and colon cancer tissue (D), showing cytoplasmic location with DAB staining using CK18 antibody.
  • Western Blot analysis using Keratin K18 antibody (10R-2034) | Western Blot showing CK18 antibody used against Hela (1), NIH/3T3 (2), A549 (3), Jurkat (4), MCF-7 (5), HepG2 (6), A431 (7), HEK293 (8) and K562 (9) cell lysate.

Availability: In stock

Price: $375.00
Size: 100 ul
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