Keratin K5 antibody (10R-2095)
Mouse monoclonal Keratin K5 antibody
|Synonyms||Monoclonal Keratin K5 antibody, Anti-Keratin K5 antibody, KRT5 antibody, Cytokeratin 5, Cytokeratin -5 antibody, Cytokeratin -5, CK5 antibody, Cytokeratin 5 antibody, Cytokeratin 5 antibody, Cytokeratin 5|
|Applications||ELISA, IHC, WB|
|Immunogen||Keratin K5 antibody was raised in Mouse using a purified recombinant fragment of CK5 expressed in E. coli as the immunogen.|
Immunohistochemical staining using Keratin K5 antibody (10R-2095)
Immunohistochemical analysis of paraffin-embedded human esophagus epithelium (A), salivary gland basal cell (B), lung squamous cell carcinoma (C), endometrium admosquamous carcinoma (D), showing cytoplasmic and membrane localization using CK5 antibody with DAB staining.
|Form & Buffer||Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Keratin K5 is a member of the keratin gene family. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Keratin K5 is expressed in normal basal cells.|
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