Keratin K5/K8 Pan Epithelial antibody (10R-2390)

Mouse monoclonal Keratin K5/K8 (Pan Epithelial) antibody

Synonyms Monoclonal Keratin K5/K8 Pan Epithelial antibody, Anti Keratin K5/K8 Pan Epithelial antibody, Keratin K/K8-5 antibody, Keratin K5/K8, Keratin K/K8 5, Keratin K/K8-5, Keratin K/K8 5 antibody, Cytokeratin 5/8 Pan Epithelial antibody
Specificity Human keratin K8
Cross Reactivity Bovine, Murine, Amphibia, Pig, Fish
Applications IHC, WB
Immunogen Keratin K5/K8 (Pan Epithelial) antibody was raised in mouse using human keratin K8, purified from SDS PAGE gel as the immunogen.

Specifications

Host Mouse
Clone C22
Isotype IgG1
Method of Purification Keratin K5/K8 (Pan Epithelial) antibody was purified by Protein A affinity chromatography
Form & Buffer Supplied in liquid form, contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 2-8 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Keratin K5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. The type II cytokeratin genes are clustered in a region of chromosome 12q12-q13. Keratin K8 is specifically expressed in the basal layer of the epidermis with family member KRT14. Antibodies to CK8 can be used to differentiate lobular carcinoma of the breast from ductal carcinoma of the breast. Keratin 8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

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