Keratin K8 antibody (10R-2036)
Mouse monoclonal Keratin K8 antibody
|Synonyms||Monoclonal Keratin K8 antibody, Anti-Keratin K8 antibody, CK8 antibody, KRT8 antibody, K8 antibody, Keratin K8, Keratin K 8, Keratin K-8, Keratin K 8 antibody, Keratin K-8 antibody|
|Applications||ELISA, IF, IHC, WB|
|Immunogen||Keratin K8 antibody was raised in Mouse using a purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. coli as the immunogen.|
Immunofluorescent staining using Western Blot analysis using Keratin K8 antibody (10R-2036) (Keratin K8 antibody)
Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using CK8 antibody (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
|Form & Buffer||Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Keratin K8 is a member of the type II keratin family. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. It typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.|
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