Keratin K8 antibody (10R-2036)

Mouse monoclonal Keratin K8 antibody

Synonyms Monoclonal Keratin K8 antibody, Anti-Keratin K8 antibody, CK8 antibody, KRT8 antibody, K8 antibody, Keratin K8, Keratin K 8, Keratin K-8, Keratin K 8 antibody, Keratin K-8 antibody
Cross Reactivity Human
Applications ELISA, IF, IHC, WB
Immunogen Keratin K8 antibody was raised in Mouse using a purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using Keratin K8 antibody (10R-2036) (Keratin K8 antibody)

Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using CK8 antibody (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.

Specifications

Host Mouse
Clone 8A5D12
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Keratin K8 is a member of the type II keratin family. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. It typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

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Images

  • Immunofluorescent staining using Western Blot analysis using Keratin K8 antibody (10R-2036) (Keratin K8 antibody) | Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using CK8 antibody (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
  • Western Blot analysis using Keratin K8 antibody (10R-2036) | Western Blot showing CK8 antibody used against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate.
  • Immunohistochemical staining using Keratin K8 antibody (10R-2036) | Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C), showing membrane and cytoplasmic localization with DAB staining using CK8 antibody.
  • Immunohistochemical staining using Keratin K8 antibody (10R-2036) | Immunohistochemical analysis of paraffin-embedded human Kidney tissues using CK8 antibody

Availability: In stock

Price: $375.00
Size: 100 ul
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