KI67 antibody (10R-1805)
Mouse monoclonal KI67 antibody
|Synonyms||Monoclonal KI67 antibody, Anti-KI67 antibody, KI 67 antibody, KI-67 antibody, KI67, KI-67, KI 67, KI 67 antibody, KI-67 antibody|
|Immunogen||KI67 antibody was raised in Mouse using synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH as the immunogen.|
Immunohistochemical staining using KI67 antibody (10R-1805)
Immunohistochemical analysis of paraffin-embedded human lymph node (A), esophagus (B), lung cancer (C), rectum cancer (D), showing nuclear localization using KI67 antibody with DAB staining.
|Form & Buffer||Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, IHC: 1:200-1:1000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Ki-67 is a nuclear protein, which is expressed in proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes.|
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