KI67 antibody (10R-2147)

Mouse monoclonal KI67 antibody

Synonyms Monoclonal KI67 antibody, Anti-KI67 antibody, KI 67 antibody, Ki-67 antibody, Ki 67, Ki-67, Ki67, KI-67 antibody, Ki 67 antibody
Cross Reactivity Human
Applications ELISA, WB
Immunogen KI67 antibody was raised in Mouse using a purified recombinant fragment of KI167(aa3118-3256) expressed in E. coli as the immunogen.

Images

Western Blot analysis using KI67 antibody (10R-2147)

Western Blot showing Ki67 antibody used against truncated Trx-Ki67 recombinant protein (1),truncated Ki67 (aa3118-3256)-His recombinant protein (2) and truncated Ki67 (aa3118-3256)-hIgGFc transfected CHO-K1 cell lysate (3).

Specifications

Host Mouse
Clone 9C12B2
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance KI67 is a nuclear protein, which is expressed in proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes.

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Images

  • Western Blot analysis using KI67 antibody (10R-2147) | Western Blot showing Ki67 antibody used against truncated Trx-Ki67 recombinant protein (1),truncated Ki67 (aa3118-3256)-His recombinant protein (2) and truncated Ki67 (aa3118-3256)-hIgGFc transfected CHO-K1 cell lysate (3).

Availability: In stock

Price: $345.00
Size: 100 ul
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