KI67 antibody (10R-2244)

Mouse monoclonal KI67 antibody

Synonyms Monoclonal KI67 antibody, Anti-KI67 antibody, KI-67 antibody, KI 67 antibody, KI67, KI 67, KI-67, KI 67 antibody, KI-67 antibody
Cross Reactivity Human
Applications ELISA, IHC
Immunogen KI67 antibody was raised in Mouse using synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH, as the immunogen.

Images

Immunohistochemical staining using KI67 antibody (10R-2244)

Immunohistochemical analysis of paraffin-embedded lung cancer (left) and rectal cancer (right) using KI67 antibody with DAB staining.

Specifications

Host Mouse
Clone 8D5
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IHC: 1:200-1:1000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance KI67 is a nuclear protein, which is expressed in proliferating cells. KI67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes.

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Images

  • Immunohistochemical staining using KI67 antibody (10R-2244) | Immunohistochemical analysis of paraffin-embedded lung cancer (left) and rectal cancer (right) using KI67 antibody with DAB staining.
  • Western Blot analysis using KI67 antibody (10R-2244) | Western Blot showing KI67 antibody used against Hela (1), MCF-7 (2) and Raji (3) cell lysate.

Availability: In stock

Price: $325.00
Size: 100 ul
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