KLHL13 antibody (10R-2206)

Mouse monoclonal KLHL13 antibody

Synonyms Monoclonal KLHL13 antibody, Anti-KLHL13 antibody, Kelch like 13 antibody, BKLHD2 antibody, BTB antibody, Kelch domain-containing protein 2 antibody, KLHL13, KLHL 13, KLHL-13, KLHL 13 antibody, KLHL-13 antibody
Cross Reactivity Human
Applications ELISA, IF, IHC, WB
Immunogen KLHL13 antibody was raised in Mouse using a purified recombinant fragment of human KLHL13 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using KLHL13 antibody (10R-2206) (KLHL13 antibody)

FC analysis of 3T3/L1 cells using KLHL13 antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 8D1
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance KLHL13 is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain. Expressed predominantly in brain, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation.

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Images

  • Flow Cytometric assay using Western Blot analysis using KLHL13 antibody (10R-2206) (KLHL13 antibody) | FC analysis of 3T3/L1 cells using KLHL13 antibody (green) and negative control (purple).
  • Western Blot analysis using KLHL13 antibody (10R-2206) | Western Blot showing KLHL13 antibody used against Hela (1) and MCF-7 (2) cell lysate.
  • Immunohistochemical staining using KLHL13 antibody (10R-2206) | Immunohistochemical analysis of paraffin-embedded brain tissues (left) and pancreas tissues (right) using KLHL13 antibody with DAB staining.
  • Immunofluorescent staining using Western Blot analysis using KLHL13 antibody (10R-2206) (KLHL13 antibody) | Immunofluorescence analysis of NTERA-2 cells (left) and U251 (right) cells using KLHL13 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Availability: In stock

Price: $345.00
Size: 100 ul
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