KLK3 antibody (10R-7924)

Mouse monoclonal KLK3 antibody

Synonyms Monoclonal KLK3 antibody, Anti-KLK3 antibody, hK3 antibody, Prostate-specific antigen antibody, APS antibody, KLK2A1 antibody, PSA antibody
Applications ELISA, WB
Immunogen KLK3 antibody was raised in Mouse using recombinant human KLK3(25-261aa) purified from E. coli as the immunogen

Images

Western Blot analysis using KLK3 antibody (10R-7924)

Extracts of PC-3 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLK3 antibody (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT1D10
Isotype IgG1 kappa
Method of Purification KLK3 antibody was purified using Protein G affinity chromatography
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. PSA is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. PSA is now used to indicate the presence of semen in forensic serology. The semen of adult males has PSA levels far in excess of those found in other tissues; therefore, a high level of PSA found in a sample is an indicator that semen may be present. Because PSA is a biomarker that is expressed independently of spermatozoa, it remains useful in identifying semen from vasectomized and azoospermic males.

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Images

  • <div><font face=Calibri size=2>Western Blot analysis using KLK3 antibody (10R-7924)</font></div>     | Extracts of PC-3 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KLK3 antibody (1:500-1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $195.00
Size: 50 ul
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