KRT19 antibody (10R-8676)
Mouse monoclonal KRT19 antibody
|Synonyms||Monoclonal KRT19 antibody, KRT19 antibody, Keratin antibody, type I cytoskeletal 19 antibody, CK19 antibody, K19 antibody, K1CS antibody|
|Immunogen||KRT19 antibody was raised in Mouse using recombinant human KRT19 (1-400aa) purified from E. coli as the immunogen|
Western Blot analysis using KRT19 antibody (10R-8676)
The cell lysates of MCF7 (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with KRT19 antibody (1:1000).
|Method of Purification||KRT19 antibody was purified by protein-A affinity chromatography|
|Form & Buffer||Supplied in liquid form in Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol and 0.02% Sodium Azide|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:1000, WB: 1:1000|
Storage & Safety
|Storage||Can be stored at 4 deg C short term. For long term storage, aliquot and store at -20 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||KRT19 (type 1 cytokeratin 19) is 44kDa intermediate filament (IF) protein that is part of the Keratin family of proteins. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation. KRT19 is an important biomarker for breast cancer cells, tumor cells of the lymph nodes, peripheral blood, and bone marrow. Cytokeratin 19 can be expressed with KRT8 and KRT18 to differentiate cells of epithelial lines from hematopoietic tumor cells in the peripheral blood.|
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