LGR5 antibody (20R-2773)

Rabbit polyclonal LGR5 antibody

Synonyms Polyclonal LGR5 antibody, Anti-LGR5 antibody, LGR 5 antibody, LGR5, LGR-5, LGR 5, Leucine-rich repeat-containing G-protein coupled receptor 5 antibody, LGR-5 antibody, GPR49 antibody, GPR49 antibody, FEX antibody
Cross Reactivity Human, Mouse
Applications WB
Immunogen LGR5 antibody was raised in Rabbit using the peptide sequence around aa 490~494(K-I-S-N-Q) derived from Human LGR5(GPR49) as the immunogen.

Images

The LGR5-transpondin pathway.

Specifications

Host Rabbit
Method of Purification LGR5 antibody was purified by immunoaffinity chromatography.
Form & Buffer Supplied in liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:500-1:1000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Product Use Polyclonal LGR5 antibody specific for Human LGR5 for use in Western Blot.
Biological Significance LGR5 is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), LGR5 associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
Expression Highly expressed in neural cells and is overexpressed in cancers of the ovary, colon and liver. In premalignant cancer expression is not restricted to the cript base. LGR5 is an integral membrane protein expressed in skeletal muscle, placenta, spinal cord, and various region of brain. LGR5 is also expressed at the base of crypts in colonic and small mucosa stem cells.
Implications in Disease LGR5 +ve stem cells were identified to fuel stem cell activity in murine intestinal adenomas via erroneous activation of the pro-cell cycle Wnt signalling pathway as a result of successive mutations, such as formation of adenoma via Adenomatous polyposis coli (APC) mutation. Loss of LGR5 actually increased tumourigenicity and invasion whereas overexpression results a reduction in tumourigenicity and clonogenicity. This implies that LGR5 is not an oncogene but a tumor suppressor gene, and that its main role is delimiting stem cell expansion in their respective niches. Varying expression profile of LGR5 was also observed in different stages of gastrointestinal cancers, which suggests that the histoanatomical distribution of LGR5+ve stem cells determine how the cancer advances.
Sequence Information LGR5 is composed of 907 amino acids with a molecular weight of 99998 Da. There is a Leucine-rich repeat-containing G-protein coupled receptor 5 chain at amino acids 22 – 907 and a signal peptide at positions 1 – 21.
Post-Translational Modifications LGR5 undergoes glycosylation2 at Asn63, Asn77, Asn208, Asn500 and Asn792.
Database Links Entrez Gene: 8549 Human, Omim: 606667 Human, SwissProt: O75473 Human, Unigene: 658889 Human

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Images

  • The LGR5-transpondin pathway.
  • Western Blot analysis using LGR5 antibody (20R-2773) | Western Blot analysis of extracts from Hela, 3T3 and HEPG2 cells using LGR5 antibody (20R-2773)

Availability: In stock

Price: $225.00
Size: 50 ug
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