LIN28 antibody (10R-2014)

Mouse monoclonal LIN28 antibody

Synonyms Monoclonal LIN28 antibody, Anti-LIN28 antibody, FLJ12457 antibody, LIN-28 antibody, LIN28A antibody, ZCCHC1 antibody, Zinc finger CCHC domain-containing protein 1 antibody, Lin 28 homolog antibody, Tex17 antibody, LIN28, LIN 28, LIN-28, LIN 28 antibody, LIN-28 antibody
Cross Reactivity Human
Applications ELISA, IF, WB
Immunogen LIN28 antibody was raised in Mouse using a purified recombinant fragment of LIN28 (aa93-209) expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using LIN28 antibody (10R-2014) (LIN28 antibody)

Confocal immunofluorescence analysis of NTERA-2 cells using LIN28 antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Specifications

Host Mouse
Clone 6D1F9
Isotype IgG1
Method of Purification LIN28 antibody was purified by protein G affinity chromatography.
Form & Buffer Supplied as a liquid in 0.01M Phosphate buffer, pH 7.4 containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance LIN28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.

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Images

  • Immunofluorescent staining using Western Blot analysis using LIN28 antibody (10R-2014) (LIN28 antibody) | Confocal immunofluorescence analysis of NTERA-2 cells using LIN28 antibody (green). Blue: DRAQ5 fluorescent DNA dye.
  • Western Blot analysis using LIN28 antibody (10R-2014) | Western Blot showing LIN28 antibody used against NTERA-2 cell lysate (1).
  • Immunofluorescent staining using Western Blot analysis using LIN28 antibody (10R-2014) (LIN28 antibody) | Confocal immunofluorescence analysis of methanol fixed Hela cells were transfected with pMX construct of human LIN28, cells were analyzed

Availability: In stock

Price: $375.00
Size: 100 ul
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