Livin beta protein (His tag) (80R-2160)
Purified recombinant Human Livin beta protein (His tag)
|Synonyms||ML-IAP protein, RNF50 protein, BIRC7 protein, MLIAP protein, KIAP protein, Livin inhibitor of apoptosis isoform beta protein|
Coomassie Blue stained SDS-PAGE of Livin beta protein (His tag) (80R-2160)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGSSHHHHHH SSGLVPRGSH MGSHMGPKDS AKCLHRGPQP SHWAAGDGPT QERCGPRSLG SPVLGLDTCR AWDHVDGQIL GQLRPLTEEE EEEGAGATLS RGPAFPGMGS EELRLASFYD WPLTAEVPPE LLAAAGFFHT GHQDKVRCFF CYGGLQSWKR GDDPWTEHAK WFPSCQFLLR SKGRDFVHSV QETHSQLLGS WDPWEEPEDA APVAPSVPAS GYPELPTPRR EVQSESAQEP GARDVEAQLR RLQEERTCKV CLDRAVSIVF VPCGHLVCAE CAPGLQLCPI CRAPVRSRVR TFLS|
|Purity||> 90% pure|
|Molecular Weight||33.4 kDa|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 300mM NaCl, 1mM EDTA|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Livin beta is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING zinc finger (RZF) domain. Livin isoform beta has direct interaction with several caspases including caspase-3, -7, and –9. This protein inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. Recombinant human Livin beta protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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