LYPLA2 protein (His tag) (80R-1684)
Purified recombinant Human LYPLA2 protein
|Synonyms||LYPLA 2 protein, APT2 protein, LPL-II protein, Lysophospholipase II protein, LYPLA2, LYPLA 2, APT-2 protein, LYPLA-2, Acyl-protein thioesterase 2 protein, LysoPLA II, LYPLA-2 protein|
Coomassie Blue stained SDS-PAGE of LYPLA2 protein (His tag) (80R-1684)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-231 amino acids: MGSSHHHHHH SSGLVPRGSH MCGNTMSVPL LTDAATVSGA ERETAAVIFL HGLGDTGHSW ADALSTIRLP HVKYICPHAP RIPVTLNMKM VMPSWFDLMG LSPDAPEDEA GIKKAAENIK ALIEHEMKNG IPANRIVLGG FSQGGALSLY TALTCPHPLA GIVALSCWLP LHRAFPQAAN GSAKDLAILQ CHGELDPMVP VRFGALTAEK LRSVVTPARV QFKTYPGVMH SSCPQEMAAV KEFLEKLLPP V|
|Purity||> 95% pure|
|Molecular Weight||26.9 kDa (251aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 10% glycerol and 100mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Acyl-protein thioesterase 2, also known LYPLA2, is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein may hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Recombinant human LYPLA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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