Lysozyme antibody (20R-LR006)

Rabbit polyclonal Lysozyme antibody

Synonyms Polyclonal Lysozyme antibody, Anti-Lysozyme antibody, EC 3.2.1.17 antibody, Lyz antibody, 1 4 beta n acetylmuramidase c antibody, LZM antibody, Lysozyme C precursor antibody, lysosyme antibody, Lysozyme C antibody
Specificity Chicken lysozyme
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, IP, WB
Immunogen Lysozyme antibody was raised in rabbit using full length protein corresponding to amino acids 1-129 of Hen Egg White lysozyme as the immunogen.

Images

Western blot analysis using Lysozyme antibody (20R-LR006)

Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane. Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4 deg C. Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated Goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: Blocking buffer overnight at 4 deg C. Size: 4.9kDa, 5kDa for Lysozyme..

Specifications

Host Rabbit
Method of Purification Lysozyme antibody was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Form & Buffer Liquid in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% Sodium Azide

Usage & Assay Information

Usage Recommendations ELISA: 1:25,000; IP: 1:100; WB: 1:500-1:2,000; IHC: 1:500-1:2,000

Storage & Safety

Storage Store at 4 deg Cfor short term storage and at-20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Lysozyme is a relatively small (129 AA) secretory enzyme that catalyzes the hydrolysis of ß-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers. Lysozyme is common in animals and plants. In birds, lysozyme is also an exceptionally abundant protein in egg whites. Its biological function in fowl eggs is unclear. Hen egg white lysozyme "c" is the most commonly studied form and source of the enzyme.

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Images

  • Western blot analysis using Lysozyme antibody (20R-LR006) | Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane. Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4 deg C. Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated Goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: Blocking buffer overnight at 4 deg C. Size: 4.9kDa, 5kDa for Lysozyme..
  • Western blot analysis using Lysozyme antibody (20R-LR006) | Western Blot of Lysozyme (Hen Egg White) antibody. Lane 1: Lysozyme (Hen Egg White). Load: 50 ng per lane. Lysozyme (Hen Egg White) antibody at 1:1,000 overnight at 4 deg C. HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).

Availability: In stock

Price: $515.00
Size: 100 ug
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