MAFG protein (His tag) (80R-2021)

Recombinant human MAFG protein (His tag)

Synonyms Transcription factor MafG protein, hMAF protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of MAFG protein (His tag) (80R-2021)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-162 amino acids: MGSSHHHHHH SSGLVPRGSH MTTPNKGNKA LKVKREPGEN GTSLTDEELV TMSVRELNQH LRGLSKEEIV QLKQRRRTLK NRGYAASCRV KRVTQKEELE KQKAELQQEV EKLASENASM KLELDALRSK YEALQTFART VARSPVAPAR GPLAAGLGPL VPGKVAATSV ITIVKSKTDA RS
Expression System E.coli
Purity > 90% pure
Molecular Weight 20.0 kDa (182aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Concentration 0.25 mg/ml

Usage & Assay Information

Usage Recommendations MAFG protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance MAFG, also known as transcription factor MafG, belongs to the Maf family. Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Recombinant human MAFG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of MAFG protein (His tag) (80R-2021) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 50 ug
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