MASH1 antibody (10R-M106b)

Ratmonoclonal MASH1 antibody

Synonyms Monoclonal MASH1 antibody, Anti-MASH1 antibody, MASH 1 antibody, Achaete-scute homolog 1 antibody, MASH-1, MASH1, MASH-1 antibody, MASH 1
Specificity Mouse MASH1
Cross Reactivity To be determined by end-user
Applications ELISA, WB
Immunogen MASH1 antibody was raised in Rat using E. coli-derived rmMash1 as the immunogen.

Images

Immunofluorescent staining using MASH1 antibody (10R-M106b)

Ascl1 staining in a subpopulation of Type-1 stem cells (arrow) and Type-2 progenitors (arrowhead) in adult hippocampus using MASH1 (Ascl1) antibody at a dilution of 1:750. Imaging was performed on a Zeiss LSM510 confocal microscope. Image taken from Kim et al, Ascl1 (Mash1) defines cells with long-term neurogenic potential in subgranular and subventricular zones in adult mouse brain; Pubmed ID: 21483754 (See reference tab)

Specifications

Host Rat
Clone 301733
Isotype IgG2a
Molecular Weight 25.4 kDa (predicted detection band MW)
Form & Buffer Lyophilized from a 0.2 u filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS.

Usage & Assay Information

Usage Recommendations ELISA: 0.5-1.0 ug/ml, WB: 1-2 ug/ml

Storage & Safety

Storage Store at -20 deg C until reconstitution. Following reconstitution product may be stored at 4 deg C in the short term. For long term storage aliquot and freeze at -20 deg C. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The ASCL1 gene, is a basic helix-loop-helix (bHLH) transcription factor with a 40 AA helix-loop-helix motif (132 to 171) and a HLH N-terminal adjacent DNA-binding 11 AA basic motif (121-131). The protein also contains closely linked 15 AA polyAla (33-47) and 12 AA polyGln (51-62) regions. BasicHLH transcription factors are heterodimeric. Mash1 is essential for neuronal differentiation and specification within the nervous system.

References
Add a Paper

Kim, E. J., Ables, J. L., Dickel, L. K., Eisch, A. J., Johnson, J

Ascl1 (Mash1) defines cells with long-term neurogenic potential in subgranular and subventricular zones in adult mouse brain

PLoS One

Issue: 6(3):e18472 PMID: 21483754 DOI: 10.1371/journal.pone.0018472

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Images

  • <div>Immunofluorescent staining using MASH1 antibody (10R-M106b)</div> | Ascl1 staining in a subpopulation of Type-1 stem cells (arrow) and Type-2 progenitors (arrowhead) in adult hippocampus using MASH1 (Ascl1) antibody at a dilution of 1:750. Imaging was performed on a Zeiss LSM510 confocal microscope. Image taken from Kim et al, Ascl1 (Mash1) defines cells with long-term neurogenic potential in subgranular and subventricular zones in adult mouse brain; Pubmed ID: 21483754 (See reference tab)

Availability: In stock

Price: $460.00
Size: 100 ug
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