MAT II beta protein (30R-1425)
Purified recombinant Human MAT II beta protein
|Synonyms||MGC12237 protein, MATIIbeta protein, # Methionine adenosyltransferase 2 beta subunit # Methionine adenosyltransferase 2 subunit beta # Methionine adenosyltransferase II beta # OTTMUSP00000005600 # Putative protein product of Nbla02999 # RP23-382C18.2 # Short chain dehydrogenase/reductase family 23E member 1 # TGR, Beta regulatory subunit of methionine adenosyltransferase protein, AI182287 protein, MAT II protein, MAT2B protein, SDR23E1 protein, TGR protein, 2410018D16Rik protein, Nbla02999 protein, dTDP 4 keto 6 deoxy D glucose 4 reductase protein, Methionine adenosyltransferase II protein, beta 1110064C04Rik protein, AU022853 protein, MAT II beta protein|
Coomassie Blue stained SDS-PAGE of MAT II beta protein (30R-1425)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-323 amino acids: MPEMPEDMEQ EEVNIPNRRV LVTGATGLLG RAVHKEFQQN NWHAVGCGFR RARPKFEQVN LLDSNAVHHI IHDFQPHVIV HCAAERRPDV VENQPDAASQ LNVDASGNLA KEAAAVGAFL IYISSDYVFD GTNPPYREED IPAPLNLYGK TKLDGEKAVL ENNLGAAVLR IPILYGEVEK LEESAVTVMF DKVQFSNKSA NMDHWQQRFP THVKDVATVC RQLAEKRMLD PSIKGTFHWS GNEQMTKYEM ACAIADAFNL PSSHLRPITD SPVLGAQRPR NTQLDCSKLE TLGIGQRTPF RIGIKESLWP FLIDKRWRQT VFH|
|Purity||> 90% pure|
|Molecular Weight||36.4 kDa (323aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Methionine adenosyltransferase II, beta (MAT II beta) belongs to the methionine adenosyltransferase (MAT) family. MAT II beta catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. This protein is the regulatory beta subunit of MAT. Its expression in hepatoma cell lines may lead to increase DNA synthesis and thereby participate to cell proliferation. Recombinant human MAT II beta was expressed in E. coli and purified by using conventional chromatography techniques.|
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