MCM2 antibody (10R-1595)

Mouse monoclonal MCM2 antibody

Synonyms Monoclonal MCM2 antibody, Anti-MCM2 antibody, MCM 2, MCM-2 antibody, KIAA0030 antibody, MGC10606 antibody, MCM-2, BM28 antibody, MITOTIN antibody, D3S3194 antibody, CDCL1 antibody, Minichromosome maintenance complex component 2 antibody, cdc19 antibody, MCM 2 antibody, MCM2, CCNL1 antibody
Specificity Human MCM2
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen MCM2 antibody was raised in mouse using recombinant Human Minichromosome Maintenance Complex Component 2 (Mcm2)

Images

Immunofluorescent staining using MCM2 antibody (10R-1595)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-MCM2 antibody.

Specifications

Host Mouse
Clone 2546C1a
Isotype IgG1
Method of Purification MCM2 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer MCM2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

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Images

  • Immunofluorescent staining using MCM2 antibody (10R-1595) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-MCM2 antibody.
  • Western Blot analysis of immunized recombinant protein using MCM2 antibody (10R-1595) | Left: MCM2 staining. Right: Coomassie Blue staining of immunized recombinant protein.

Availability: In stock

Price: $190.00
Size: 50 ug
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