MCTS1 protein (His tag) (80R-2699)
Purified recombinant MCTS1 protein (His tag)
|Synonyms||Malignant T cell amplified sequence 1 protein, MCT-1 protein, MCT1 protein, MCTS1, MCTS 1, MCTS-1, MCTS 1 protein, MCTS-1 protein|
SDS-PAGE analysis of MCTS1 protein (His tag) (80R-2699)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-181 aa: MGSSHHHHHH SSGLVPRGSH MGSMFKKFDE KENVSNCIQL KTSVIKGIKN QLIEQFPGIE PWLNQIMPKK DPVKIVRCHE HIEILTVNGE LLFFRQREGP FYPTLRLLHK YPFILPHQQV DKGAIKFVLS GANIMCPGLT SPGAKLYPAA VDTIVAIMAE GKQHALCVGV MKMSAEDIEK VNKGIGIENI HYLNDGLWHM KTYK|
|Purity||> 95% pure|
|Molecular Weight||22.9 kDa|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT|
Storage & Safety
|Storage||Can be stored at 4 deg C for short terms (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles|
|Biological Significance||Malignant T cell amplified sequence 1, also known as MCTS1, plays a role as translation enhancer; Recruits the density-regulated protein/DENR and binds to the cap complex of the 5'-terminus of mRNAs, subsequently altering the mRNA translation profile; Up-regulates protein levels of BCL2L2, TFDP1, MRE11A, CCND1 and E2F1, while mRNA levels remains constant. Recombinant human MCTS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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