MDM4 antibody (10R-1842)

Mouse monoclonal MDM4 antibody

Synonyms Monoclonal MDM4 antibody, Anti-MDM4 antibody, MDM-4 antibody, MDM 4 antibody, MDM4, MDM 4, MDM-4, MDM 4 antibody, MDM-4 antibody
Cross Reactivity Human
Applications ELISA, IF, IHC, WB
Immunogen MDM4 antibody was raised in Mouse using a purified recombinant fragment of human MDM4 expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using MDM4 antibody (10R-1842) (MDM4 antibody)

Confocal immunofluorescence analysis of Hela (left) and L-02 (right) cells using MDM4 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Specifications

Host Mouse
Clone 2D10F4
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance MDM4 contains a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2.

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Images

  • Immunofluorescent staining using Western Blot analysis using MDM4 antibody (10R-1842) (MDM4 antibody) | Confocal immunofluorescence analysis of Hela (left) and L-02 (right) cells using MDM4 antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
  • Western Blot analysis using MDM4 antibody (10R-1842) | Western Blot showing MDM4 antibody used against Hela (1), A549 (2) and A431 (3) cell lysate.
  • Immunohistochemical staining using MDM4 antibody (10R-1842) | Immunohistochemical analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using MDM4 antibody.
  • Immunohistochemical staining using MDM4 antibody (10R-1842) | Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using MDM4 antibody

Availability: In stock

Price: $345.00
Size: 100 ul
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