MDM4 antibody (70R-10492)

Affinity purified rabbit polyclonal MDM4 antibody

Synonyms Polyclonal MDM4 antibody, Anti-MDM4 antibody, DKFZp781B1423 antibody, MDM4, MDM-4, MDM-4 antibody, MRP1 antibody, transformed 3T3 cell double minute 4, MGC132766 antibody, Mdm4, p53 binding protein, MDM 4, mouse antibody, MDM 4 antibody, MDMX antibody
Cross Reactivity Human
Applications WB
Immunogen MDM4 antibody was raised in rabbit using the N terminal of MDM4 as the immunogen
Assay Information MDM4 Blocking Peptide, catalog no. 33R-4100, is also available for use as a blocking control in assays to test for specificity of this MDM4 antibody

Images

Western blot analysis using MDM4 antibody (70R-10492)

Recommended MDM4 Antibody Titration: 0.2-1 ug/ml

Specifications

Host Rabbit
Form & Buffer Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-1 ug/ml

Storage & Safety

Storage Store at 4 deg C, following reconstitution, aliquot and store at -20 deg C. Avoid repeated freeze-thaw cycles.

General Information

Biological Significance MDM4 inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. It inhibits degradation of MDM2. It can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2.

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Images

  • Western blot analysis using MDM4 antibody (70R-10492) | Recommended MDM4 Antibody Titration: 0.2-1 ug/ml
  • Western blot analysis using MDM4 antibody (70R-10492) | Lanes: 1. 10 ug MCF7 cell lysate 2. 10 ug MCF7 cell lysate 3. 10 ug MCF7 cell lysate 4. 10 ug MCF7 cell lysate stained with MDM4 antibody at  1:1000

Availability: In stock

Price: $345.00
Size: 50 ug
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