MEK2 antibody (10R-8476)

Mouse monoclonal MEK2 antibody

Synonyms Monoclonal MEK2 antibody, Anti-MEK2 antibody, MAP2K2 antibody, mitogen-activated protein kinase kinase 2 antibody, BOS_7688 antibody, dual specificity mitogen activated protein kinase kinase 2 antibody, dual specificity mitogen-activated protein kinase kinase 2 antibody, ERK activator kinase 2 antibody, MAPK/ERK kinase 2 antibody, MAP kinase/Erk kinase antibody, MAP kinase kinase 2 antibody, MAPKK2 antibody, MAPKK 2 antibody, MEK2 antibody, MEK 2 antibody, mitogen activated protein kinase kinase 2 antibody, mitogen-activated protein kinase kinase 2 antibody, p45 antibody, mitogen-activated protein kinase kinase type 2 antibody, MK2 antibody, MKK2 antibody, PRKMK2 antibody, protein kinase antibody, mitogen activated antibody, kinase 2 antibody, p45 antibody
Cross Reactivity Human
Applications IP, WB
Immunogen MEK2 antibody was raised in Mouse using a recombinant human protein purified from E.coli (His-MEK2) as the immunogen

Images

Western Blot analysis using MEK2 antibody (10R-8476)

Specifications

Host Mouse
Clone 1A1
Isotype IgG1 kappa
Method of Purification MEK2 antibody was purified by ammonium sulfate precipitation
Form & Buffer Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Usage & Assay Information

Usage Recommendations WB: 1: 2000, IP: 2 ul

Storage & Safety

Storage Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles

General Information

Biological Significance The ERK (extracellular-signal regulated kinase) cascade is a central MAPK pathway which contains Ras as a G protein, Raf as a MAPKKK, MEK1 (MAPK/ERK kinase1) and MEK2 (MAPK/ERK kinase2) as MAPKK, and ERKs as MAPK. MEK1 and MEK2 are 80% identical to each other, and are essentially identical in most of their kinase domain. MEK1 and MEK2 phosphorylate ERKs equally well, both in vivo and in vitro. MEK1 (45 kDa) and MEK2 (46 kDa) are composed of a catalytic kinase domain, which is surrounded by a regulatory N-terminal domain ( 80 amino acids) and a shorter C-terminal region ( 30 amino acids). Unlike the kinase domains, the N-termini and the characteristic Pro-rich inserts are quite divergent between the two MEKs (40% identity). MEKs are activated by phosphorylation of two Ser residues in their activation loop (Ser218 and Ser222 in MEK1) located within a Ser?Xaa?Ala?Xaa?Ser/Thr motif, typical to all MAPKKs.

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Images

  • Western Blot analysis using MEK2 antibody (10R-8476) |

Availability: In stock

Price: $295.00
Size: 100 ul
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