Methyllysine antibody (20R-2837)

Rabbit polyclonal Methyllysine antibody

Synonyms Polyclonal Methyllysine antibody, MLY antibody, MLZ antibody, Methylated Lysine antibody
Applications ELISA, IHC, IP, WB
Immunogen Methyllysine antibody was raised in Rabbit using methylated lysine-KLH conjugate as the immunogen

Images

Western Blot analysis using Methyllysine antibody (20R-2837)

Western blot analysis of Methylated Lysine in BSA (left) and Methylated BSA (right).

Specifications

Host Rabbit
Form & Buffer Supplied in PBS buffer, pH 7.0 and 50% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1-10 ug/ml, IHC: 1-10 ug/ml, WB: 0.5-1 ug/ml

General Information

Biological Significance In proteins, the amino acid residue lysine can be methylated once, twice or thrice on its terminal sidechain ammonium group. Such methylated lysines play an important role in epigenetics; the methylation of specific lysines of certain histones in a nucleosome alters the binding of the surrounding DNA to those histones, which in turn affects the expression of genes on that DNA. The binding is affected because the effective radius of the positive charge is increased (methyl groups are larger than the hydrogen atoms they replace), reducing the strongest potential electrostatic attraction with the negatively charged DNA. Moreover, the methyl groups are themselves hydrophobic, and alter the structure of water in their vicinity, similar to tetramehtyl ammonium (Jencks reference).

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Images

  • <div><font face=Calibri size=2>Western Blot analysis using Methyllysine antibody (20R-2837)</font></div>     | Western blot analysis of Methylated Lysine in BSA (left) and Methylated BSA (right).

Availability: In stock

Price: $245.00
Size: 200 ul
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