Mimitin protein (His tag) (80R-1889)

Purified recombinant Mimitin protein

Synonyms Mimitin protein, NDUFA12L protein, MMTN protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of Mimitin protein (His tag) (80R-1889)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-169 amino acids: MGSSHHHHHH SSGLVPRGSH MGWSQDLFRA LWRSLSREVK EHVGTDQFGN KYYYIPQYKN WRGQTIREKR IVEAANKKEV DYEAGDIPTE WEAWIRRTRK TPPTMEEILK NEKHREEIKI KSQDFYEKEK LLSKETSEEL LPPPVQTQIK GHASAPYFGK EEPSVAPSST GKTFQPGSWM PRDGKSHNQ
Expression System E.coli
Purity > 85% pure
Molecular Weight 22kDa (189aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 20% glycerol and 200mM NaCl.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance NDUFAF2, also known as Mimitin, belongs to the complex I NDUFA12 subunit family. NADH dehydrogenase is an enzyme located in the inner mitochondrial membrane that catalyzes the transfer of electrons from NADH to coenzyme Q (CoQ). It is the 'entry enzyme' of oxidative phosphorylation in the mitochondria. Mimitin protein acts as a molecular chaperone for mitochondrial complex I assembly. Recombinant human NDUFAF2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of Mimitin protein (His tag) (80R-1889) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $340.00
Size: 50 ug
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