MIS12 protein (His tag) (80R-2632)

Purified recombinant Human MIS12 protein (His tag)

Synonyms MTW 1 protein, MIS-12, MIS 12 protein, MIS12, KNTC2AP protein, MTW1 protein, MTW-1 protein, Protein MIS12 homolog protein, hMis12 protein, MIS-12 protein, MIS 12
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of MIS12 protein (His tag) (80R-2632)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-205 aa: MGSSHHHHHH SSGLVPRGSH MGSMSVDPMT YEAQFFGFTP QTCMLRIYIA FQDYLFEVMQ AVEQVILKKL DGIPDCDISP VQIRKCTEKF LCFMKGHFDN LFSKMEQLFL QLILRIPSNI LLPEDKCKET PYSEEDFQHL QKEIEQLQEK YKTELCTKQA LLAELEEQKI VQAKLKQTLT FFDELHNVGR DHGTSDFRES LVSLVQNSRK LQNIRDNVEK ESKRLKIS
Expression System E.coli
Method of Purification MIS12 protein was purified using Protein G affinity chromatography
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 2M Urea, 10% glycerol
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Protein MIS12 homolog, also known as MIS12, is a component of the MIS12 complex, which is required for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex consists of MIS12, DSN1, NSL1 and PMF-1. MIS12 is part of a network of complexes that provide microtubule attachment and generates pulling forces from depolymerization. Recombinant human MIS12 protein, fused to His-tag at N-terminus, was expressed in E.coli.

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Images

  • <div><font face=Calibri size=2>SDS-PAGE analysis of MIS12 protein (His tag) (80R-2632)</font></div>     | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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