MLH1 antibody (10R-1841)

Mouse monoclonal MLH1 antibody

Synonyms Monoclonal MLH1 antibody, Anti-MLH1 antibody, Mutl Homolog 1 Colon Cancer Nonpolyposis Type 2 antibody, COCA2 antibody, FCC2 antibody, HNPCC antibody, HNPCC2 antibody, MGC5172 antibody, hMLH1 antibody antibody, MLH1, MLH 1, MLH-1, MLH 1 antibody, MLH-1 antibody
Cross Reactivity Human, Monkey
Applications ELISA, IF, IHC, WB
Immunogen MLH1 antibody was raised in Mouse using a purified recombinant fragment of MLH1 (aa381-483) expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using MLH1 antibody (10R-1841) (MLH1 antibody)

Confocal immunofluorescence analysis of Hela cells using MLH1 antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Specifications

Host Mouse
Clone 4C9C7
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral part of the protein complex responsible for mismatch repair expressed inlymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid andgall bladder, and is methylated in several ovarian tumors.

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Images

  • Immunofluorescent staining using Western Blot analysis using MLH1 antibody (10R-1841) (MLH1 antibody) | Confocal immunofluorescence analysis of Hela cells using MLH1 antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western Blot analysis using MLH1 antibody (10R-1841) | Western Blot showing MLH1 antibody used against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.
  • Immunohistochemical staining using MLH1 antibody (10R-1841) | Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 antibody.

Availability: In stock

Price: $345.00
Size: 100 ul
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