MSH2 antibody (10R-1791)

Mouse monoclonal MSH2 antibody

Synonyms Monoclonal MSH2 antibody, Anti-MSH2 antibody, MSH 2, MSH2, MSH 2 antibody, MSH-2 antibody, MSH-2
Cross Reactivity Human, Monkey
Applications ELISA, IF, IHC, WB
Immunogen MSH2 antibody was raised in Mouse using a purified recombinant fragment of human MSH2 expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using MSH2 antibody (10R-1791) (MSH2 antibody)

Confocal immunofluorescence analysis of Hela cells using MSH2 antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Specifications

Host Mouse
Clone 1B3=3A2B8C
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.

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Images

  • Immunofluorescent staining using Western Blot analysis using MSH2 antibody (10R-1791) (MSH2 antibody) | Confocal immunofluorescence analysis of Hela cells using MSH2 antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western Blot analysis using MSH2 antibody (10R-1791) | Western Blot showing MSH2 antibody used against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.
  • Immunohistochemical staining using MSH2 antibody (10R-1791) | Immunohistochemical analysis of paraffin-embedded human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 antibody with DAB staining.

Availability: In stock

Price: $295.00
Size: 100 ul
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