MSRB3 protein (His tag) (80R-1709)
Purified recombinant Human MSRB3 protein
|Synonyms||MSRB-3 protein, MSRB 3, protein, MSRB-3, MSRB 3 protein, MSRB3, Methionine sulfoxide reductase B3 protein|
Coomassie Blue stained SDS-PAGE of MSRB3 protein (His tag) (80R-1709)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||21-185 amino acids: MCGLPSGSCR DKKNCKVVFS QQELRKRLTP LQYHVTQEKG TESAFEGEYT HHKDPGIYKC VVCGTPLFKS ETKFDSGSGW PSFHDVINSE AITFTDDFSY GMHRVETSCS QCGAHLGHIF DDGPRPTGKR YCINSAALSF TPADSSGTAE GGSGVASPAQ ADKAELLEHH HHHH|
|Purity||> 90% pure|
|Molecular Weight||19 kDa (174aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 10% glycerol and 0.1mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Methionine sulfoxide reductase B3, also known as MSRB3, belongs to the methionine sulfoxide reductases (MSR) family proteins. MSRs are thought to protect against reactive oxygen species-induced oxidative damage in many organs, including the most environmentally exposed organ, human skin. MSRB3 plays an important role in cold tolerance by eliminating MetO and ROS that accumulate at the ER during cold acclimation. Recombinant human MSRB3 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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