MUG protein (His tag) (80R-2762)

Purified recombinant MUG protein (His tag)

Synonyms G/U mismatch-specific DNA glycosylase protein, xanthine DNA glycosylase protein, dug protein, ECK3058 protein, JW3040 protein, ygjF protein, MUG
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of MUG protein (His tag) (80R-2762)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-168 aa: MGSSHHHHHH SSGLVPRGSH MGSMVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R
Expression System E.coli
Purity > 90% pure
Molecular Weight 21.1 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol
Concentration 500 ug/ml

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance G/U mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as MUG, belongs to the TDG/MUG DNA glycosylase family. It has been proposed that the MUG protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Recombinant E.coli MUG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • SDS-PAGE analysis of MUG protein (His tag) (80R-2762) | Figure annotation denotes ug of protein loaded and % gel used.
  • SDS-PAGE analysis of MUG protein (His tag) (80R-2762) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 50 ug
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