MYL5 protein (His tag) (80R-1983)

Recombinant human MYL5 protein (His tag)

Synonyms MYL5, MYL-5, MYL-5 protein, MYL 5 protein, MYL 5, Myosin light chain 5 Isoform1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of MYL5 protein (His tag) (80R-1983)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-173 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMASRKT KKKEGGALRA QRASSNVFSN FEQTQIQEFK EAFTLMDQNR DGFIDKEDLK DTYASLGKTN VKDDELDAML KEASGPINFT MFLNLFGEKL SGTDAEETIL NAFKMLDPDG KGKINKEYIK RLLMSQADKM TAEEVDQMFQ FASIDVAGNL DYKALSYVIT HGEEKEE
Expression System E.coli
Purity > 90% pure
Molecular Weight 22.1 kDa(197aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations MYL5 protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance Myosin regulatory light chain 5, also known as MYL5, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. Reconstitution of myosin with regulatory light chain 5 or alkali light chain increases filament velocity to intermediate rates, and readdition of both classes of light chains fully restores the original sliding velocity. Recombinant human MYL5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of MYL5 protein (His tag) (80R-1983) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 50 ug
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