Myoglobin antibody (10R-3136)

Mouse monoclonal Myoglobin antibody

Synonyms Monoclonal MB antibody, Anti-MB antibody PVALB antibody, PVALB antibody, MB antibody
Specificity Human
Cross Reactivity Mouse
Applications ELISA, WB
Immunogen Myoglobin antibody was raised in mouse using recombinant human MB protein purified from E.coli as the immunogen.

Images

Western Blot analysis of Myoglobin antibody (10R-3136)

The extracts of mouse muscle and heart (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT6E10
Isotype IgG1 kappa
Method of Purification Myoglobin antibody was purified by protein G affinity chromatography
Form & Buffer Supplied in liquid form in PBS (pH 7.4) with 0.1% Sodium Azide.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles..

General Information

Biological Significance Myoglobin is an an iron- and oxygen-binding protein. It is found in abundance in the muscle tissue of vertebrates in general and in almost all mammals. MB (myglobin) is related to hemoglobin, which consists of four myoglobin-like subunits that form a tetramer and are responsible for carrying oxygen in blood. The only time myoglobin is found in the bloodstream is when it is released following muscle injury. It is an abnormal finding, and can be diagnostically relevant when found in blood.

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Images

  • Western Blot analysis of Myoglobin antibody (10R-3136) | The extracts of mouse muscle and heart (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $270.00
Size: 100 ul
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