Myoglobin antibody (10R-3136)
Mouse monoclonal Myoglobin antibody
|Synonyms||Monoclonal MB antibody, Anti-MB antibody PVALB antibody, PVALB antibody, MB antibody|
|Immunogen||Myoglobin antibody was raised in mouse using recombinant human MB protein purified from E.coli as the immunogen.|
Western Blot analysis of Myoglobin antibody (10R-3136)
The extracts of mouse muscle and heart (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
|Method of Purification||Myoglobin antibody was purified by protein G affinity chromatography|
|Form & Buffer||Supplied in liquid form in PBS (pH 7.4) with 0.1% Sodium Azide.|
Usage & Assay Information
|Usage Recommendations||WB: 1:1000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles..|
|Biological Significance||Myoglobin is an an iron- and oxygen-binding protein. It is found in abundance in the muscle tissue of vertebrates in general and in almost all mammals. MB (myglobin) is related to hemoglobin, which consists of four myoglobin-like subunits that form a tetramer and are responsible for carrying oxygen in blood. The only time myoglobin is found in the bloodstream is when it is released following muscle injury. It is an abnormal finding, and can be diagnostically relevant when found in blood.|
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