Nanog antibody (10R-2069)

Mouse monoclonal Nanog antibody

Synonyms Monoclonal Nanog antibody, Anti-Nanog antibody, Homeobox protein NANOG antibody, hNanog antibody, Nanog antibody, Embryonic stem cell specific homeobox protein antibody, Homeobox transcription factor Nanog antibody, Embryonic stem cell specific homeobox protein antibody, ENK antibody, Anti Nanog antibody, hNanog antibody, ENK antibody, Homeobox transcription factor Nanog antibody, Homeobox protein NANOG antibody
Cross Reactivity Human
Applications ELISA, IF, WB
Immunogen Nanog antibody was raised in Mouse using a purified recombinant fragment of Nanog (aa20-166) expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using Nanog antibody (10R-2069) (Nanog antibody)

Immunofluorescence analysis of human iPS cells using NANOG antibody (green).

Specifications

Host Mouse
Clone 1E6C4
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The transcriptional factor Nanog functions in maintaining pluripotency in cooperation with other key genes such as Oct4. Oct4 and Sox2 bind to the Nanog promoter in mouse and human embryonic stem cell. Nanog levels allow stem cells to balance the creation of lineage-committed and undifferentiated cells.

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Images

  • Immunofluorescent staining using Western Blot analysis using Nanog antibody (10R-2069) (Nanog antibody) | Immunofluorescence analysis of human iPS cells using NANOG antibody (green).
  • Western Blot analysis using Nanog antibody (10R-2069) | Western Blot showing NANOG antibody used against NTERA-2 cell lysate (2).
  • Immunofluorescent staining using Western Blot analysis using Nanog antibody (10R-2069) (Nanog antibody) | Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Availability: In stock

Price: $345.00
Size: 100 ul
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