NANS antibody (10R-3129)
Mouse monoclonal TPMT antibody
|Synonyms||Monoclonal TPMT antibody, Anti-TPMT antibody Sialic acid synthase antibody, SAS antibody, N acetylneuraminate synthase antibody, N acetylneuraminate synthase antibody, SAS antibody, TPMT antibody, Anti TPMT antibody Sialic acid synthase antibody|
|Immunogen||NANS antibody was raised in mouse using recombinant human NANS (1-359aa) purified from E. coli as the immunogen.|
Western Blot analysis of NANS antibody (10R-3129)
The cell lysates (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NANS antibody(1:100, 1:1000, 1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
|Method of Purification||NANS antibody was purified by protein G affinity chromatography.|
|Form & Buffer||Supplied as a liquid in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide|
Usage & Assay Information
|Usage Recommendations||WB: 1:1000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NANS is an enzyme that functions in the biosynthetic pathways of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis.|
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