NAPG protein (His tag) (80R-1984)

Recombinant human NAPG protein (His tag)

Synonyms GAMMA-SNAP protein, Gamma-soluble NSF attachment protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of NAPG protein (His tag) (80R-1984)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-312 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMAAQKI NEGLEHLAKA EKYLKTGFLK WKPDYDSAAS EYGKAAVAFK NAKQFEQAKD ACLREAVAHE NNRALFHAAK AYEQAGMMLK EMQKLPEAVQ LIEKASMMYL ENGTPDTAAM ALERAGKLIE NVDPEKAVQL YQQTANVFEN EERLRQAVEL LGKASRLLVR GRRFDEAALS IQKEKNIYKE IENYPTCYKK TIAQVLVHLH RNDYVAAERC VRESYSIPGF NGSEDCAALE QLLEGYDQQD QDQVSDVCNS PLFKYMDNDY AKLGLSLVVP GGGIKKKSPA TPQAKPDGVT ATAADEEEDE YSGGLC
Expression System E.coli
Purity > 95% pure
Molecular Weight 37.3 kDa(336aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations NAPG protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance NAPG, also known as gamma-SNAP, is a cytoplasmic protein that binds to a membrane receptor complex composed of VAMP, SNAP25 and syntaxin. It mediates the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP25 and syntaxins for VAMP binding. Recombinant human NAPG protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of NAPG protein (His tag) (80R-1984) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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