NARS antibody (10R-1221)

Mouse monoclonal NARS antibody

Synonyms Monoclonal NARS antibody, Anti-NARS antibody, NARS antibody, Asparagine tRNA ligase antibody, Anti NARS antibody, ASNRS antibody, Asparagine tRNA ligase antibody, ASNRS antibody
Specificity Human NARS
Cross Reactivity Human, Mouse. Other species not studied
Applications Dot Blot, ICC, IP, WB
Immunogen NARS antibody was raised in mouse using recombinant Asparaginyl-Trna Synthetase

Images

Immunofluorescent staining using NARS antibody (10R-1221)

Immunostaining analysis in HT1080 cells. HT1080 cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-NARS antibody.

Specifications

Host Mouse
Clone NARS2G5
Isotype IgG1
Method of Purification NARS antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer NARS antibody in PBS (3.0 mM KCl, 1.5 mM KH2PO4 , 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, IP: 100-500 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Aminoacy1-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginy1-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginy1-tRNA synthetases.

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Images

  • Immunofluorescent staining using NARS antibody (10R-1221) | Immunostaining analysis in HT1080 cells. HT1080 cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-NARS  antibody.
  • Western Blot analysis of immunized recombinant protein using NARS antibody (10R-1221)

Availability: In stock

Price: $190.00
Size: 50 ug
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