NCEH1 protein (His tag) (80R-2706)
Purified recombinant NCEH1 protein (His tag)
|Synonyms||Neutral cholesterol ester hydrolase 1 protein, AADACL1 protein, NCEH protein, NCEH1, NCEH 1, NCEH-1, NCEH 1 protein, NCEH-1 protein|
SDS-PAGE analysis of NCEH1 protein (His tag) (80R-2706)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-275 aa: MGSSHHHHHH SSGLVPRGSH MGSMAEELNA VIVSIEYRLV PKVYFPEQIH DVVRATKYFL KPEVLQKYMV DPGRICISGD SAGGNLAAAL GQQFTQDASL KNKLKLQALI YPVLQALDFN TPSYQQNVNT PILPRYVMVK YWVDYFKGNY DFVQAMIVNN HTSLDVEEAA AVRARLNWTS LLPASFTKNY KPVVQTTGNA RIVQELPQLL DARSAPLIAD QAVLQLLPKT YILTCEHDVL RDDGIMYAKR LESAGVEVTL DHFEDGFHGC MIFTSWPTNF SVGIRTRNSY IKWLDQNL|
|Purity||> 90% pure|
|Molecular Weight||33.6 kDa|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol|
Storage & Safety
|Storage||Can be stored at 4 deg C for short terms (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles|
|Biological Significance||Neutral cholesterol ester hydrolase 1, also known as NCEH1, hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. It may be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also NCEH1 is involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. This protein may contribute to cancer pathogenesis by promoting tumor cell migration. Recombinant human NCEH1 protein, fused to His-tag at N-terminus, was expressed in E.coli.|
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