NCK1 protein (His tag) (80R-1350)
Purified recombinant Human NCK1 protein
|Synonyms||NCK protein, NCK 1 protein, NCKalpha protein, SH2/SH3 adaptor protein NCK alpha protein, NCK1, beta protein, SH2/SH3 adaptor protein NCK beta., NCK tyrosine kinase protein, non catalytic region of tyrosine kinase protein, NCK-1, NCKbeta protein, NCK 1, MGC12668 protein, NCK adaptor protein 1 GRB4 protein, NCK-1 protein, noncatalytic region of tyrosine kinase protein, melanoma NCK protein protein, NCK adaptor protein 2 protein|
Coomassie Blue stained SDS-PAGE of NCK1 protein (His tag) (80R-1350)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-377 amino acids: MGSSHHHHHH SSGLVPRGSH MAEEVVVVAK FDYVAQQEQE LDIKKNERLW LLDDSKSWWR VRNSMNKTGF VPSNYVERKN SARKASIVKN LKDTLGIGKV KRKPSVPDSA SPADDSFVDP GERLYDLNMP AYVKFNYMAE REDELSLIKG TKVIVMEKCS DGWWRGSYNG QVGWFPSNYV TEEGDSPLGD HVGSLSEKLA AVVNNLNTGQ VLHVVQALYP FSSSNDEELN FEKGDVMDVIEKPENDPEWW KCRKINGMVG LVPKNYVTVM QNNPLTSGLE PSPPQCDYIR PSLTGKFAGN PWYYGKVTRH QAEMALNERG HEGDFLIRDS ESSPNDFSVS LKAQGKNKHF KVQLKETVYC IGQRKFSTME ELVEHYKKAP IFTSEQGEKL YLVKHLS|
|Purity||> 90% pure|
|Molecular Weight||45 kDa (397aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 20% glycerol, 1mM EDTA, and 50mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NCK adaptor protein 1, also known as NCK, belongs to the adaptor family of proteins. The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast. Recombinant human NCK1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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