NDK protein (His tag) (80R-2168)
Purified recombinant E.coli NDK protein (His tag)
|Synonyms||Nucleoside diphosphate kinase protein, ECK2514 protein, JW2502 protein|
Coomassie Blue stained SDS-PAGE of NDK protein (His tag) (80R-2168)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGSSHHHHHH SSGLVPRGSH MGSHMAIERT FSIIKPNAVA KNVIGNIFAR FEAAGFKIVG TKMLHLTVEQ ARGFYAEHDG KPFFDGLVEF MTSGPIVVSV LEGENAVQRH RDLLGATNPA NALAGTLRAD YADSLTENGT HGSDSVESAA REIAYFFGEG EVCPRTR|
|Purity||> 95% pure|
|Molecular Weight||18.0 kDa|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NDK, is an enzyme that catalyzes the exchange of phosphate groups between different nucleoside diphosphates. This protein activity maintains an equilibrium between the concentrations of different nucleoside triphosphates such as, for example, when GTP produced in the citric acid (Krebs) cycle is converted to ATP. Recombinant E. coli ndk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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