NEIL1 protein (His tag) (80R-2089)

Recombinant human NEIL1 protein (His tag)

Synonyms NEIL 1 protein, NEIL-1 protein, NEIL-1, FPG1 protein, NEI1 protein, Endonuclease 8-like 1 protein, NEIL 1, NEIL1, hFPG1 protein, FLJ22402 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of NEIL1 protein (His tag) (80R-2089)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-390 amino acids: MGSSHHHHHH SSGLVPRGSH MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA LQQHRPSPEL TLSQKIRTKL QNPDLLELCH SVPKEVVQLG GKGYGSESGE EDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRP EGTSLQQDPE APTVPKKGRR KGRQAASGHC RPRKVKADIP SLEPEGTSAS
Expression System E.coli
Purity > 90% pure
Molecular Weight 45.8 kDa (410aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. Recombinant human NEIL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of NEIL1 protein (His tag) (80R-2089) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 10 ug
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